lab Report Format Give the specific enzyme, chemical equation, parameters (values), tool and actual results in one paragraph (II) Introduction (30 points) (enzyme) What specific enzyme was used? What was the specific chemical reaction did the enzyme work on (need actual chemical equation)? [Biological background] Which organism has this enzyme? [Biologic What valuable function did this enzyme provided to the organism? What instrument did you use to measure the enzyme activity? How exactly did you use this instrument to measure enzyme activity? {parameters} What are the four main parameters and all specific values (of those parameters) that you’re going to test in terms of the enzyme? What specific value for each parameter did you predict would be best for enzyme activity?
(III) Materials & Methods (20 points) Explain why you have 2 test tubes to start the experiment Why did you preincubate empty tubes at different temperatures. Was the inhibitor competitive or non-competitive? What wavelength you used? List the specific values for each parameter. List all the step-by-step instructions. (IV) Results (20 points) What is the main title of your graph? Label the X and Y axes of the graph. Label the actual graphed lines Give a short description of what values and condition you are measuring. Give the highest and lowest absorbance values for each specific condition for each graph. (V) Conclusion (15 points) What are the specific conditions that gave the best peroxidase enzyme efficiency? Explain why those conditions were best for peroxidase. Explain why the other conditions did NOT work. How do they compare to your predictions? (VI) References (5 points) List your textbook, lab manual and other sources, such as websites.
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