Attached are three pictures
* Gram stained sample under 100x objective
* Phenol Red Fermentation tubes (sucrose, lactose, glucose)
* Streak for Isolation plate (Nutrient agar)
1. You will compare your results to the unknown key provided in lab materials (also attached to pre-lab 11)
* I recommend completing pre-lab 11 before the unknown project to practice using the unknown key
* Your results should match one of the organisms on the unknown key
* Not all results will match PERFECTLY, but there should be one organism that fits the best
2. Once you have determined your organism it is time to begin your report
* The report is worth 20 points split into 7 categories
Use the rubric and statements below to determine what should be on your report:
Technique explanation (3 pts)
* Describe how you would perform a Gram stain
* Describe how you would perform a Streak for Isolation
1. Unknown number (1 pt)
* Include your assigned unknown number
2. Data chart (2 pts)
* Copy the style of the data chart provided on the unknown key
* The chart should have 3 rows by 5 columns
* The first row should contain the title of each column (again copying the style of the unknown key data table)
* The second row should be your expected results for the bacteria you identified. This should have the name of your organism and the exact results stated on the unknown key (basically copy the results from the unknown key for your identified organism)
* The third row should be your interpretation of the results provided for you in the three images.
* This may match the second row exactly, it may not
* There should be no “V” or variable in the third row, variable means it could be anything. You saw the results so you should have an answer.
* In this row, you should also include your specific visual observations of colors of the different tests.
Terminology (2 pts)
* Communicates scientific concepts and terminology using appropriate symbols, notation and vocabulary
* Use proper binomial nomenclature
* Properly capitalizes Gram stain
* Uses proper terminology to describe their cell morphology/arrangement
Results (4 pts)
* Describe the results for each test indicated below. Use full sentences and write your answers in proper paragraph form.
* Gram stain
* Positive or Negative (How did you know?)
* Cell arrangement
* Cell morphology
* Phenol Red Fermentation
* Did fermentation occur in each sugar?
* What products were produced? (How could you tell?)
* Streak for Isolation
* Successful or unsuccessful? (How could you tell?)
* Pigmented or non-pigmented?
* Can you see/describe any other characteristics of Colony Morphology?
 Analyze (5 pts)
* Correctly identify which organism you have
* Analyzes, evaluates, and interprets the results obtained
* Justifies how the results and observations were used to determine which organism you had
* Basically, walk through the thought process/critical thinking steps you used to determine which organism you had. If some of the provided results did not quite match up with the expected results for your chosen bacteria, you should discuss how those results may have impaired your ability to determine which bacteria you had.
 Discussion (3 pts) – Required to include citations for sources of information used in this section.
* Provide a thorough written description of additional characteristics of your organism
* Location of where that bacteria is commonly found
* Whether that bacteria causes infection to humans and the type of infection it can cause
* You can include any relevant/interesting information you find about your bacteria
* You can also relate your bacteria back to our in class testing (Did we use it in class? Is it catalase positive? Does it hydrolyze casein?